![]() ![]() ![]() Four classifier proteins, WD repeat-containing protein 63, amyloid-like protein 1, SPARC-like protein 1, and cell adhesion molecule 3, were identified by feature selection and externally validated. ![]() We then used our CSF proteomic profiles to create a support vector machines classifier capable of discriminating training set ALS from non-ALS (HC and OND) samples. Enrichment analysis was used to link these alterations to biological pathways, which were predominantly related to inflammation, neuronal activity, and extracellular matrix regulation. Levels of several proteins with diverse biological functions were significantly altered in sALS samples. A total of 1712 CSF proteins were detected and relatively quantified by spectral counting. To identify new protein/pathway alterations and candidate biomarkers for amyotrophic lateral sclerosis (ALS), we performed comparative proteomic profiling of CSF from sporadic ALS (sALS), healthy control (HC), and other neurological disease (OND) subjects using label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS). Label-Free LC-MS/MS Proteomic Analysis of Cerebrospinal Fluid Identifies Protein/Pathway Alterations and Candidate Biomarkers for Amyotrophic Lateral Sclerosis.Ĭollins, Mahlon A An, Jiyan Hood, Brian L Conrads, Thomas P Bowser, Robert PĪnalysis of the cerebrospinal fluid (CSF) proteome has proven valuable to the study of neurodegenerative disorders. ![]()
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